Isolation and Restriction Endonuclease Analysis of Mycobacterial DNA
نویسندگان
چکیده
منابع مشابه
Restriction endonuclease analysis of Aspergillus fumigatus DNA.
AIMS To develop a genome based DNA fingerprinting system for Aspergillus fumigatus mould. METHODS DNA was extracted from 21 isolates obtained from eight patients with an aspergilloma. This was with a freeze-dried mycelial extract fragmented in liquid nitrogen. DNA was subsequently purified by phenol-chloroform extraction followed by ultracentrifugation on a caesium chloride gradient. The DNA ...
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SseBI, an isoschizomer of StuI (1) has been purified from Streptomyces species. SseBI recognises the sequence 5' ... AGGCCT ... 3' and cleaves between G and C. The enzyme was purified using the following chromatographic steps: 1. Blue Sepharose F3GA, 2. Heparin-Sepharose. The enzyme was free of contaminating nuclease activity. After 100 fold overdigestion on lambda DNA greater than 95% of the D...
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Tuberculosis (TB) is one of the most important AIDS associated infectious diseases worldwide. It is a leading cause of illness and death among people with HIV/AIDS in resource-poor areas of the world. The annual incidence of TB among indigenous Iranians stands at 14 cases per 100,000 inhabitants. This study aimed to identify Mycobacterium infection among Iranian HIV positive patients. Two sputu...
متن کاملIsolation, characterization and restriction endonuclease mapping of the Petunia hybrida chloroplast DNA.
A procedure is developed for the isolation of intact chloroplast DNA (ctDNA) from Petunia hybrida. The molecular weight, calculated from contour length measurements, is 96.0 +/- 4.5 x 10(6) daltons. This value is in good agreement with the value of 101.2 x 10(6) daltons that was estimated from the electrophoretic mobilities of restriction endonuclease fragments of ctDNA. Analysis of petunia ctD...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1986
ISSN: 1350-0872,1465-2080
DOI: 10.1099/00221287-132-2-541